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Protective effect of DNA-mediated immunization with a combination of SAG1 and IL-2 gene adjuvant against infection of Toxoplasma gondii in mice

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Author:
No author available
Journal Title:
CHINESE MEDICAL JOURNAL
Issue:
10
DOI:
10.3760/cma.j.issn.0366-6999.2002.10.103
Key Word:
DNA免疫 弓形虫 表面抗原(SAG1) 基因佐剂 IL-2;DNA immunization;Toxoplasma gondi;surface antigen 1 (SAG1);gene adjuvant;IL-2

Abstract: Objective To characterize the immune response induced by SAG1 encoding plasmid combined with IL-2 gene adjuvant in mice and to assess the protective effect of this vaccination against toxoplasmosis.Methods Mice were co-injected intramuscularly with plasmid encoding Toxoplasma gondii SAG1 plus murine IL-2 expression vector at a d ose of 100 μg. Booster immunizations were employed 2 more times at 3-week in terval. As controls, mice were inoculated with PBS or empty plasmid pcDNA3. Hu moral and cellular responses were assayed using ELISA for the determination of Ab, Ab isotype and IFN-γ, as well as IL-4. To detect the integration and diss emination of DNA in the injected mice, PCR and in situ hybridization were perfor med. All mice were then infected with highly virulent RH tachyzoites of Toxopla sma gondii intraperitoneally.Results Significant increases in specific IgG levels were obser ved in mice after immunization three times with SAG1 expression plasmid. With r espect to the IgG isotype, co-inoculation of IL-2 expression plasmid enhanced the level of IgG2a and the production of IFN-γ. Challenging mice by vaccinat ing with combined plasmids with RH tachyzoites resulted in prolonged survival. Conclusion Humoral and cytokine responses elicited by SAG1 DNA immunization can be modulated by co-inoculation with IL-2 expression plasmid. The use of DNA vaccine in combination with an appropriate cytokine gene to prev ent T. gondii infection warrants further investigation.

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