Novel mitochondrial 16S rRNA mutation, 3200T→C, associated with adult-onset type 2 diabetes
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.0366-6999.2002.05.123
- Key Word:
- 二型糖尿病 线粒体DNA 16S 核糖体RNA RNA二级结构模拟;type 2 diabetes;mitochondrial DNA;16S ribosomal RNA;RNA secondary structure modeling
Abstract： Objective To investigate the role of a potential diabetes-related mitochondrial region, w hich includes two previously reported mutations, 3243AG and 3316GA, in Chine se patients with adult-onset type 2 diabetes.Methods A total of 277 patients and 241 normal subjects were recruited for the study. M itochondrial nt 3116-3353, which spans the 16S rRNA, tRNAleu(UUR) and the NADH dehydrogenase 1 gene, were detected using polymerase chain reaction (PCR), direct DNA sequencing, PCR-restriction fragment length p olymorphism and allele-specific PCR. Variants were analyzed by two-tailed Fis her exact test. The function of the variants in 16S rRNA were predicted for mi nimal free energy secondary structures by RNA folding software mfold version 3.
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