Prognostic value of matrix metalloproteinase-2 and tissue inhibitor of metall oproteinase-2 in bladder carcinoma
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 5
- DOI:
- 10.3760/j.issn:0366-6999.2002.05.021
- Key Word:
- 膀胱移行细胞癌;金属基质蛋白酶;金属基质蛋白酶组织抑制物;bladder transitional cell carcinoma;matrix metalloproteinase-2;tissue inhibitor of metalloproteinase-2
Abstract： Objectives To detect the level of matrix metalloproteinase-2 (MMP-2) mRNA and the tissue inhibitor of metalloproteinase-2 (TIMP-2) mRNA in bladder transitional cell ca rcinoma (BTCC), and to estimate the prognosis for bladder tumor based on the qua lity and quantity of MMP-2 and TIMP-2 mRNA.Methods Thirty-five samples of human BTCC and 15 normal fresh bladder tissues were stud ied by RT-PCR analysis followed by computer-assisted image analysis.Results The level of the MMP-2 mRNA in BTCC was significantly increased compared with t hat in normal bladder epithelium. The positive rates of MMP-2 and TIMP-2 mRNA were 71.4% and 65.7% in BTCC, and 66.7% and 60.0% in the normal bladder wal l. The expression intensity of the MMP-2 mRNA by image analysis tended to incr ease with tumor grading and staging, which showed statistical significance. Sim ilarly, the MMP-2 to TIMP-2 ratio also showed statistically significant differ ence between normal bladder tissue and bladder carcinoma (P<0.01).Conclusions A high level of the MMP-2 mRNA exists in BTCC, which may function to damage col lagen Ⅳ inside the basement membrane and the extracellular basement of the blad der. The level of the MMP-2 mRNA is proportional to BTCC grading and staging, which may have prognostic value. The MMP-2 to TIMP-2 ratio may play a more si gnificant role in the determination of the aggressiveness and prognosis of bladd er tumor.
- [1]B L, Lokeshwar.MMP inhibition in prostate cancer.[J].1999,878.
- [2]B, Davies,J, Waxman,H, Wasan,etc.Levels of matrix metalloproteases in bladder cancer correlate with tumor grade and invasion.[J].1993,53.
- [3]Kanayama Hiro\u2010omi,Yokota Kin\u2010ya,Kurokawa Yasushi,etc.Prognostic values of matrix metalloproteinase\u20102 and tissue inhibitor of metalloproteinase\u20102 expression in bladder cancer[J].1998.
- [4]Jung K,Ulbrich N,Loening SA,etc.Quantification of matrix metalloproteinases and tissue inhibitors of metalloproteinase in prostatic tissue: analytical aspects.[J].1998,34(2).
- [5]M A, Moses,D, Wiederschain,K R, Loughlin,etc.Increased incidence of matrix metalloproteinases in urine of cancer patients.[J].1998,58.
- [6]B L, Lokeshwar,M G, Selzer,N L, Block,etc.Secretion of matrix metalloproteinases and their inhibitors (tissue inhibitor of metalloproteinases) by human prostate in explant cultures: reduced tissue inhibitor of metalloproteinase secretion by malignant tissues.[J].1993,53.
- [7]Nishitani M,Tsuji M,Kanda K,etc.Role of the matrix metalloproteinase and tissue inhibitors of metalloproteinase families in noninvasive and invasive tumors transplanted in mice with severe combined immunodeficiency.[J].1998,51(5).
- [8]W C, Powell,J D, Knox,M, Navre,etc.Expression of the metalloproteinase matrilysin in DU-145 cells increases their invasive potential in severe combined immunodeficient mice.[J].1993,53.
- [9]Maurizio Onisto,Maria Pia Riccio,Paolo Scannapieco,etc.gelatinase A/TIMP-2 imbalance in lymph-node-positive breast carcinomas, as measured by RT-PCR[J].1995,63(5).