Role of angiotensin Ⅱand angiotensin Ⅱ receptors in vascular smooth muscle cell migration in vitro
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 5
- DOI:
- 10.3760/j.issn:0366-6999.2002.05.002
- Key Word:
- 血管紧张素Ⅱ;血管平滑肌细胞;细胞;受体;迁移;angiotensin Ⅱ;vascular smooth muscle;cells;receptor;migration
Abstract： Objective To determine the biotic effects of angiotensin Ⅱ (Ang Ⅱ) on the migration of r at smooth muscle cells (VSMCs) and investigate the mechanisms involved in the d evelopment of vascular injury. Methods VSMCs isolated from aortic media of Wistar rats and cultured by the modified exp lant method were adopted. In the presence and absence of Ang Ⅱ, the expressio n of Ang Ⅱ receptor (ATR) and reorganization of the actin cytoskeleton and foca l adhesion of VSMCs were studied by an immunocytochemistry technique and fluoroc ytochemistry technique. Migration assays were performed with a modified Boyden' s chamber. The effects of AT1R antagonist (CV-11974), AT2R antagonist (PD 123319) on the aforementioned target were studied.Results VSMCs migration was stimulated by adding Ang Ⅱ. The dynamic reorganization of actin cytoskeleton and focal adhesions may be an important mechanism by which Ang Ⅱ facilitates VSMCs motility. The expression of AT1R in VSMCs could be upregulated initially after treatment with Ang Ⅱ, then decreased gradually. Th e expression of AT1R was downregulated by AT1R antagonists. The effect of Ang Ⅱ on VSMCs migration was mediated by AT1R, while AT2R had no signific ant effect. Conclusions The dynamic reorganization of focal adhesions and the actin cytoskeleton is requ ired for Ang Ⅱ-induced VSMCs migration. This effect is mediated by AT1R.
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