Expression of positive and negative regulators of cell cycle during wound healing
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.0366-6999.2002.03.102
- Key Word:
- 伤口愈合;细胞周期;循环素;循环素依赖激酶;循环素依赖激酶抑制物;wound healing cell cycle cyclin cyclin dependent kinase cyclin-dependent kinase inhibitors
Abstract： Objective To detect the expression of cell cycle positive regulators cyclin D1, cyclin E, CDK2, CDK4 and negative regulators p21cip1, p27kip1, p16ink4a and p15ink4b during wound healing in rats. Methods Open wounds of full-thickness skin, diameter 1.8?cm, on rat backs were used as the wound model. Wound tissues were harvested on postwounding days 3, 5, 7, 9, 11, 14, 21 and 30. Ki67 expression in granulation tissue was detected by immunohistochemical assay. The patterns of the expression of cyclin D1, cyclin E, CDK2, CDK4 and p21cip1, p27kip1, p16ink4a, p15ink4b were detected by Western blot. Results Cell proliferation in granulation tissue took place predominantly within the first week after injury, with the proliferation peak occurring at postwounding day 5. There were no dramatic variations in the expression of cyclin D1, CDK2 and CDK4 during wound healing. Up-regulated cyclin E was maintained from day 3 to 11 after injury, and then was down-regulated. No expression of p16ink4a and p15ink4b was found. p21cip1 was expressed only from day 7 to 14, with peak expression observed on day 9. Constitutive p27kip1 was expressed throughout wound healing with low levels in the proliferating period of day 3 to 5 and with increased levels in the post-mitotic and remodeling stage. The expression of p21cip1 and p27kip1 showed an inverse gradient to that of Ki67.Conclusion p21cip1 and p27kip1 play a supervising role in preventing the hyperproliferative tendency in tissue repair.
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