Effect of puerarin on the expression of pigment epithelium-derived factor in the rat diabetic retinopathy

( views:279, downloads:0 )
Author:
SONG Han(Department of Ophthalmology Heilongjiang Provincial Hospital,Harbin 150036,China)
TENG Yan()
YANG Ming-ming()
Journal Title:
Journal of Chinese Physician
Issue:
Volume 14, Issue 09, 2012
DOI:
10.3760/cma.j.issn.1008-1372.2012.09.012
Key Word:
PUERARIN/therapeutic use;Diabetic retinopathy/ZHONG YAO LIAO FA;Nerve growth factors/metabolism;Rats

Abstract: Objective To investigate the effect of puerarin on the expression of pigment epithelium-derived factor (PEDF) in the retina of diabetic rats.Methods The diabetic retinopathy rat model was established.Rats were randomly divided into three groups (normal,diabetic,and diabetic with puerarin injection).The pathological changes of the retinopathy were examined.PEDF was analyzed with PCR in different time point.Results Body weight of diabetic group [ ( 216.9 ± 8.37 ) g,and(179.1 ±7.56)g] was compared with normal group[ (298.2 -±6.78)g,and(323.5 -±6.45)g] in the 3 month and 5 month ( q =12.39,P <0.01 ; q =15.47,P <0.01 ).Blood glucose of diabetic group and diabetic with puerarin injection group was compared with normal group at each time point ( P < 0.01,P < 0.05 ).HE staining of the retina of diabetic rats indicated a diabetic retinopathy occurred in the early - stage in diabetic group,but changed slightly in the diabetic model with an injection of puerarin.The PEDF expression at the 5 month time point in normal group,diabetic group,and diabetic group with puerarin injection was 8.833 (1.200),1.650(3.300),and 8.350( 1.100),respectively.Compared with normal group and diabetic group with puerarin injection,the level of PEDF was significantly decreased in diabetic group (x2 =48.57,P < 0.01 ;x2=48.46,P < 0.01 ).Conclusions Puerarin can alleviate the retinal pathological damage of diabetic rats,early intervention with puerarin can prevent from the expression of PEDF and delay the diabetic retinopathy process.

  • [1]郑彦,过贵元.胰岛素在糖尿病视网膜病变中的研究进展.国际眼科杂志,2012,2(3):461-463.
  • [2]Klein R,Klein BE,Moss SE.The Wisconsin Epidemiologic Study of Diabetic Retinopathy.XVI.The relationship of C-peptide to the incidence and progression of diabetic retinopathy. Diabetes.1995,144(7):796-801.
  • [3]杨杰,彭辉灿,陈倩,等.雷公藤红素对高糖环境下大鼠视网膜血管内皮细胞VEGF和PEDF表达的影响.眼科新进展,2011,31(4):321-323,331.
  • [4]任远,张锡林.色素上皮细胞源性因子的抗肿瘤活性.生命的化学,2011,31 (6):844-848.
  • [5]冯霞,刘哲丽.PEDF与视网膜新生血管.国际眼科杂志,2006,6(5):1120-112.
  • [6]Ogata N,Tombran-Tink J,Nishikawa M,et al.Pigment epithelium-derived factor in the vitreous is low in diabetic retinopathy and high in rhegmatogenous retinal detachment.Am J Ophthalmol,2001,132(3):378-382.
  • [7]刘宁宁,才娜,陈蕾.色素上皮衍生因子的表达与早期糖尿病大鼠视网膜血管形态学的改变.眼科新进展,2012,32(2):105-108.
  • [8]韩春妍.葛根素治疗相关疾病的研究.实用药物与临床,2012,15(3):178-180.
  • [9]姚鹏,杨惠婷,滕岩,等.葛根素对糖尿病视网膜病变中Bcl-2表达的影响.国际眼科杂志,2011,11(5):788-791.
  • [10]Yao Y,Guar M,Zhao XQ,et al.Downregulation of the pigment epithelium derived factor by hypoxia and elevated glucose concentration in cultured human retinal pigment epithelial cells.Zhonghua Yi Xue Za Zhi,2003,83 (22):1989-1992.
  • [11]Eichler W,Yafai Y,Wiedemann P,et al.Angiogenesis-related factors derived from retinal glial (Müller) cells in hypoxia.Neuroreport,2004,15(10):1633-1637.
  • [12]Aymerich MS,Alberdi EM,Martinez A,et al.Evidence for pigment epithelium-derived factor receptors in the neural retina.Invest Ophthalmol Vis Sci,2001,42(13):3287-3293.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn