The temporal and spatial expression patterns of adipsin, a candidate gene for diabetic nephropathy

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Author:
WANG Xiao-lei(Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China)
FAN Qiu-ling(Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China)
WANG Li-ning(Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China)
YAO Li(Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China)
LIU Xiao-dan(Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China)
MA Jian-fei(Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China)
FENG Jiang-min(Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China)
Journal Title:
Journal of Chinese Physician
Issue:
Volume 14, Issue 09, 2012
DOI:
10.3760/cma.j.issn.1008-1372.2012.09.001
Key Word:
Diabetic nephropathies/genetics;Diabetes mellitus,Type 2/genetics;Mice

Abstract: Objective With a GcneChip(R) cxpression analysis,98 known gencs and 31 exprcssed sequence tags (ESTs) were found to be differentially expressed between KK/Ta and BALB/c kidneys.To further screen the susceptibility genes for diabetic nephropathy,the temporal and spatial expression patterns of differentially expressed gene-adipsin were investigated.Methods The body weight,blood glucose,urinary albumin/creatinine ratio,and renal pathological changes of KK/Ta and BALB/c mice were measured at the 7,20,28 and 36 weeks of age.Total RNA was extracted from the kidney,heart,liver,lung,and brain.The temporal and spatial expression patterns of adipsin in diabetic KK/Ta mice were examined by competitive RT-PCR.The correlation analysis between adipsin expression and albuminuria level was carried out.Results The mRNA expression of adipsin was found in the kidney,heart,lung,and brain,but not in liver.The expression of adipsin in diabetic KK/Ta mice at 20 weeks of age was significantly down-regulated in kidney,heart,and lung than that in age-matched BALB/c mice,and unaltered in brain.Adipsin expression in KK/Ta kidneys was significantly down-regulated with aging and negatively correlated to urinary albumin/creatinine ratio( r =-0.807,P < 0.05).Conclusions The expression of adipsin mRNA was downregulated in kidney,heart,and lung in diabetic state.Adipsin expression in KK/Ta kidneys was negatively correlated to urinary albumin/creatinine ratio.It might be a candidate gene for diabetic nephropathy.

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