Abstract： Objective To investigate the expression of Twist protein in adenocarcinoma of prostate and clinicopathological features and explore its roles in the carcinogenesis and progression of adenocarcinoma of prostate and its relationship with prognosis.Methods The expression of Twist was detected in 70 cases with adenocarcinoma of prostate and 30 cases with side cancer tissue by immunohistochemistry (EnlivionTM).Results The expression of Twist protein in adenocarcinoma of prostate was higher than side cancer tissue.The expression of twist was related to Gleason grade,clinical stage and bony metastasis (P＜0.05),but it was not related to the age (P＞0.05).The survival rate of Twist protein positive patients was significant lower than negative patients(x2=9.52,P＜0.01).Conclusions Twist might be an important biological marker for malignant transformation and invasion and metastasis adenocarcinoma of prostate,and the up-regulation of the expression of Twist reflects more invasion and poor prognosis.