The use of laser capture microdissection in the research of nephrology
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2009.03.005
- Key Word:
- 激光;显微切割/方法;膜糖蛋白类/生物合成;肌动蛋白类/生物合成;肾小球;肾小管;基因表达;Lasers;Microdissection/MT;Membrane glycoproteins/BI;Actins/BI;Kidney glomerulus;Kidney tubules;Gene expression
Abstract： Objective To explore how to use laser capture microdissection(LCM)to collect glomerulus.Methods LCM was used tocollect the normal glomerulus,renal mbes and glomerula.real-time PCR(real-time polymerase chain reaction)was used to detect the speeificity gene of glomerula nephrin expression at the glomerulus and renal tubes.Western-blotting was used to detect Actin expression at glomerulus.Results The results of LCM/real-time PCR revealed that the expression of nephrin gene in the glomerulus was higher than that in renal tubes (P＜0.05).Actin expression at glomeruhs can be easily detected by LCM/Western-blotting.Conclusions Glomernlus can be rapidly,simply and fully collected by LCM.The quality of the collected glomerulus is suitable for real-time PCR and Western-blotting.
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