The use of laser capture microdissection in the research of nephrology

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XU Hui(Xiangya Hospital,Central South University,Changsha 410008,China)
TANG Rong(Xiangya Hospital,Central South University,Changsha 410008,China)
Tao Li-jian(Xiangya Hospital,Central South University,Changsha 410008,China)
Margetts J Peter()
Journal Title:
Volume 11, Issue 03, 2009
Key Word:
Lasers;Microdissection/MT;Membrane glycoproteins/BI;Actins/BI;Kidney glomerulus;Kidney tubules;Gene expression

Abstract: Objective To explore how to use laser capture microdissection(LCM)to collect glomerulus.Methods LCM was used tocollect the normal glomerulus,renal mbes and glomerula.real-time PCR(real-time polymerase chain reaction)was used to detect the speeificity gene of glomerula nephrin expression at the glomerulus and renal tubes.Western-blotting was used to detect Actin expression at glomerulus.Results The results of LCM/real-time PCR revealed that the expression of nephrin gene in the glomerulus was higher than that in renal tubes (P<0.05).Actin expression at glomeruhs can be easily detected by LCM/Western-blotting.Conclusions Glomernlus can be rapidly,simply and fully collected by LCM.The quality of the collected glomerulus is suitable for real-time PCR and Western-blotting.

  • [1]许辉,孙明,陶市坚,等.转化生长因子-β1在自发性高血压大鼠肾脏的表达及与肾间质纤维化的关系.中华高血压杂志,2004,12(1):50-54.
  • [2]Bupérez M,Sánchez-López E,Blance-Colio al.The Rho-kinase pathway regulates AngII-induced renal damage Kidney Int,2005,68[Supp 99]
  • [3]Robert F.Bonnet,Michael Emmert-Buck,Kristina Cole,et al.LaserCapture Microdissection:Molecular Analysis of Tissue.Science,1997,(278)5342:1481-1483.
  • [4]Takada Y,Fariss RN,Tanikawa A,et al.Aminal neuronal developmental wave of retinoschisin expression begins in ganglion cells during layer formation.Invest Ophthalmol Vis Sci,2005,5:3302-3312.
  • [5]Shimamura M,Garcia JM,Prough DS,et al.Analysis of long-term gene expression in neurons of the hippocampal subfields following brain injury rats.Neuroseience,2005,31:87-97.
  • [6]Whetsell L,Maw G,Nation N,et al.Polymerase chain reaction microanalysis of tumors from stained histological slides.Oncogene,1992,7:2355-2361.
  • [7]Yukiamasa Kohda,Hiroshi Murakami,Orson W.Moe,et al.Analysis of segmental renal gene expression by laser capture micredissection.Kidney International,2000,57(1):321-331.
  • [8]Pinzani P,Salvadori B,Simi L,et al.Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer:correlation with real-time reverse transcriptase-polymerase chain reaction resuits and feasibility of molecular analysis by laser microdissection.Hum Pathol,2006,(6):711-718.
  • [9]Immuno-LCM.Laser Capture Microdissection of Immunostained Frozen Sections for mBNA Analysis.American Journal of Pathology,1999,154:61-66.
  • [10]Buckanovich HJ,Sasaroli D,Obrien-Jenkins A,et al.Use of immuno-LCM to identify the in situ expression profile of cellular constituents of the tumor microenvironment.Cancer Biol Ther,2006,5(6):635-642.
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