The use of laser capture microdissection in the research of nephrology

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Author:
XU Hui(Xiangya Hospital,Central South University,Changsha 410008,China)
TANG Rong(Xiangya Hospital,Central South University,Changsha 410008,China)
Tao Li-jian(Xiangya Hospital,Central South University,Changsha 410008,China)
Margetts J Peter()
Journal Title:
JOURNAL OF CHINESE PHYSICIAN
Issue:
Volume 11, Issue 03, 2009
DOI:
Key Word:
Lasers;Microdissection/MT;Membrane glycoproteins/BI;Actins/BI;Kidney glomerulus;Kidney tubules;Gene expression

Abstract: Objective To explore how to use laser capture microdissection(LCM)to collect glomerulus.Methods LCM was used tocollect the normal glomerulus,renal mbes and glomerula.real-time PCR(real-time polymerase chain reaction)was used to detect the speeificity gene of glomerula nephrin expression at the glomerulus and renal tubes.Western-blotting was used to detect Actin expression at glomerulus.Results The results of LCM/real-time PCR revealed that the expression of nephrin gene in the glomerulus was higher than that in renal tubes (P<0.05).Actin expression at glomeruhs can be easily detected by LCM/Western-blotting.Conclusions Glomernlus can be rapidly,simply and fully collected by LCM.The quality of the collected glomerulus is suitable for real-time PCR and Western-blotting.

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