Influence of jiaweibugan decoction on the expression of JNK mitogen-activated protein kinase in sciatic nerve of experimental diabetic rats
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-1372.2009.02.005
- Key Word:
- 补益肝肾;汤剂;糖尿病神经病变/中药疗法;坐骨神经;p38丝裂原活化蛋白激酶类/生物合成;Reforeing liver & kidney;Decoctions;Diabetic neuropathies/ZD;Sciatic nerve;p38 mitogen-activated protein kinase
Abstract： Objective To observe the effect of jiaweibugan decoction on the expression of p-JNK mitogen-activated protein kinase in sciatic nerve of experimental diabetic rats and explore the preventive and therapeutic effects of Jiaweibugan decoction on peripheral neuropa-thy in experimental diabetic rats. Methods The diabetic rat model was established by streptozotocin (STZ). The rats were killed on the 4th or 8th week from the beginning of treatment, and the expression of p-JNK mitogen-activated protein kinase in sciatic nerve was detected by immunohistochemistry. Serum SOD was determined by xanthine oxidasemethod. Results The immunohistochemistry results showed that the expression of p-iNK in the model group on the 4th or 8th week was higher than that in normal control group (P<0.05) , while p-JNK in jiaweibugan decoction group was markedly lower than that in model rats(P<0.05). The results showed that SOD in the model group on the 4th or 8th week was lower than that in the normal control group (P<0.05) , while SOD in jiaweibugan decoction group was markedly high-er than that in model rats(P<0.05). Conclusion Jiaweibugan decoction has a preventive and therapeutic effect on peripheral neuropathy in experimental diabetic rats.
- [1]陈泽奇.加味补肝汤治疗糖尿病周围神经病变[J].2003,(27).
- [2]Wada R,Baba M,Nukada H,etc.Prolonged ischemic conduction failure after reperfusion in diabetic nerve.[J].2006,33(3).
- [3]M, Brownlee.Biochemistry and molecular cell biology of diabetic complications.[J].2001,414(6865).
- [4]梅全喜,毕焕新.现代中药药理手册[M].1998.
- [5]J. Sylvester,W. Q. Li,M. Ahmad,etc.Molecular mechanisms of N-acetylcysteine actions[J].2003,60(1).
- [6]Patel J,Averill SA,Tomlinson DR,etc.Aberrant neurofilament phosphorylation in sensory neurons of rats with diabetic neuropathy.[J].1999,48(4).
- [7]Eva L, Feldman.Oxidative stress and diabetic neuropathy: a new understanding of an old problem.[J].2003,111(4).
- [8]Yun CH,Yoon SO,Chung AS.Dose effect of oxidative stress on signal transduction in aging.[J].2002,123(12).
- [9]Grodsky GM,Evans JL,Maddux BA,etc.Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes.[J].2002,23(5).
- [10]阴健(中医),郭力弓(医学).中药现代研究与临床应用[M].1993.
- [11]黄泰康 施诚.中药方剂现代研究大典[M].1996.
- [12]Inoshita S,Umezawa A,Hatai T,etc.Phosphorylation and inactivation of myeloid cell leukemia 1 by JNK in response to oxidative stress.[J].2002,277(46).
- [13]Batinic-Haberle I,Benov L.A Manganese porphyrin suppresses oxidative stress and extends the life span of streptozotocin-diabetic rats[J].2005,39(1).
- [14]Schmeichel AM,Schmelzer JD,Low PA.Oxidative injury and apoptosis of dorsal root ganglion neurons in chronic experimental diabetic neuropathy.[J].2003,52(1).