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Investigation of differential gene expression in rat myocardial stunning with cDNA microarry

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Author:
No author available
Journal Title:
JOURNAL OF CHINESE PHYSICIAN
Issue:
10
DOI:
10.3760/cma.j.issn.1008-1372.2008.10.005
Key Word:
心肌顿抑/遗传学;寡核苷酸序列分析;基因;基因表达;Myocardial stunningc/GE;Oligonucleotide array sequence analysis;Genes;Gene expression

Abstract: Objective To investigate the differential gene expression of myocardial stunning in rats, and provide basis for further studying the mechanism of myocardial stunning with cDNA microarry. Methods The model of myocardial stunning in rats was established.27K Rat Genome Array was used to examine and analyze the differentially expressed genes of stunned myocardium tissues with the sham oper-ated control myocardium tissues. Results There were 292 genes in stunned myocardium tissues with two folds differential expression, in which 199 genes were up- regulated and 93 genes were down-regulated. The up- regulated genes include H SPT0, Aft3, Gadd45b, Egr2,Vergeand et al, and the highest up-regulation amplitude is 23.1 folds. Their function were involved in anti- apoptosis, blood vessel and nerve growth, transcription, survival, recovery and et al. The function of most down-regulated gene were unknown. Conclusions Myocardi-al stunning may protect isehemic myocardium through up - regulating some cardioprotective genes. It may be a novel treatment target for is-chemic myocardial disease in the future through improving the expression of these cardioprotective genes.

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