Detection of 11q23 deletion and trisomy 12 in chronic lymphocytic leukemia by interphase fluorescence in situ hybridization
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1008-1372.2008.10.001
- Key Word:
- 白血病;淋巴细胞,慢性;遗传学;染色体缺失;原位杂交,荧光;Leukemia,lymphocytic,ehronic/GE;Chromosome deletion;In situ hybridization,fluorescence
Abstract： Objective To investigate the incidence of trisomy 12(+12) and 11 q23 deletion [ del ( 11q23) ] in chronic lymphocyticleukemia (CLL). Methods Fluorescein labeled DNA probe 12 and sequence specific probe ATM for 11q23 were used to perform inter-phase fluorescence in situ hybridization (I-FISH) assays in 30 patients with CLL. The results were compared with that of conventional cyto-genetic (CC) examination. Results With CC examination , only 4 cases (13.3%) were found to have chromosomal abnormalities, whereaswith I-FISH assay ,8 cases (26.7%) were found to have genomic aberrations, including trisomy 12 in 5 cases , deletion of 11q23 in 3 ca-sea. Conclusion I-FISH is a useful method for detection of genomie aberration in CLL, the significance of trisomy 12 and del (11q23) inpredicting the prognosis of B-CLL need to be investigated further.
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