You Position: Home > Paper

Influence of dexamethasone on airway inflammation and CD4+ CD25+ regulatory T cells of asthmatic rats

( views:227, downloads:12 )
Author:
No author available
Journal Title:
JOURNAL OF CHINESE PHYSICIAN
Issue:
8
DOI:
10.3760/cma.j.issn.1008-1372.2008.08.003
Key Word:
地塞米松/药理学;哮喘;炎症;T淋巴细胞;CD4阳性T淋巴细胞;Dexamethasone/PD;Asthma;Inflammation;T-lymphocytes;CD4-positive T-lymphocytes

Abstract: Objective To investigate dexamethasone on airway inflammation and CD4+ CD25 + regulatory T cells (CD4+ CD25 +Tr) of asthmatic rats,and elucidate the possible mechanism of dexamethasone in treatment of asthma.Methods 30 Wistar rats were randomly divided into control group,asthma group and dexamethasone-treated group.Bronchoalveolar lavage fluid (BALF) was collected,and cytology study was conducted.The lung tissue was obtained and pathologic analysis was done through HE stain.Flow eytometry was used to detect the CD4+ CD25 +Tr ratio in PBMCs.Results Total cells number,the percentage of lymphocytes,neutrophils and eosinophils (Eos)in BALF of dexamethasone-treated group were lower than that of asthma group (P<0.05,P<0.01).Compared with the asthma group,less infiltration of inflammatory cells in lung tissues was observed in the dexamethasone-treated group.CD4+ CD25 + Tr of asthma group was lower than that of control and dexamethasone-treated group (P<0.05).Conclusion Dexamethasone could suppress airway inflammation of asthmatic rats,which probably be due to increasing the number of CD4+ CD25 + Tr.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn