The expression of GSTπ in the HepG2 cell after multiple thermotherapy
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2008.03.004
- Key Word:
- 高温,诱发;肝肿瘤;谷胱甘肽转移酶;Hyperthermia,induced;Liver neoplasms;Glutathione transferase
Abstract： Objective To investigate the expression change of ghtathione S-transferase π(GSTπ)protein and mRNA in the HepG2 cell after multiple thermotherapy.Methods HepG2 cells were treaded by ten repeated cycles of exposure at 43 degree C for 80 minutes twice a day，the sensibility of HepG2 cell to Adriamycin was analyzed by MTr assay，and the expression of QSTπprotein and mRNA were detected by immunohistochemical method and RT-PCR.Results The drug resistance to Adriamycin was gained by HepG2 cells after multiple thermotherapy，and the expression of GSTπ protein and mRNA wag strengthened.Conclusions The resistance of heated HepG2 cells to chemotherapeutics was concerned with overexpression of GSTπ protein and mRNA.
- [1]秦春宏,李永国,杨文军,等.重复加热对肝癌HepG2细胞多药耐药相关蛋白表达的影响[J].2007,(6).
- [2]Lou YJ,Zhang J.Relationship between activation of microsomal glutathione S-transferase and metabolism behavior of chlorambucil.[J].2003,48(6).
- [3]Parody JP,Alvarez-Mde L,Quiroga A,etc.Hepatocytes isolated from preneoplastic rat livers are resistant to ethacrynic acid cytotoxicity.[J].2007,81(8).
- [4]A, Hevér-Szabó,M, Pirity,M, Szathmári,etc.P-glycoprotein is overexpressed and functional in severely heat-shocked hepatoma cells.[J].1900,18(4C).