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Observation on biological characteristics of human adrenocortical carcinoma SW-13 cell lines cultured in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2007.03.004
- Key Word:
- 肾上腺皮质肿瘤
Abstract： 目的 探讨人肾上腺皮质癌SW-13细胞株的培养方法及生物学特性.方法 将SW-13细胞株进行体外培养,MTT法检测其生长曲线,电镜下、光镜下进行细胞形态学观察.结果 细胞需在37℃,无CO2,偏碱性环境下培养,6～8h完全贴壁,2d左右进入对数生长期.细胞生物学性状稳定.结论 SW-13细胞株是一种生物学特性稳定、实用的细胞株,相信随着研究的进一步深入,其必将发挥更大的作用价值,有着更广的研究领域.
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