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The study of the source and the cultivation of the smooth muscle cells and the endothelial cells for the tissue engineering bile duct
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.12.002
- Key Word:
- 胆管;肌细胞,平滑肌;内皮细胞;支架
Abstract： 目的 解决组织工程化胆管的支架材料及细胞来源(1)体外培养人胚动脉平滑肌细胞(VSMCs),并观察人胚VSMCs在乳酸与羟基乙酸共聚物(PLGA)上的三维培养情况.(2)探讨体外诱导大鼠骨髓间充质干细胞( rBMSCs)定向分化为肝干细胞的可行性.方法 (1)成功培养人胚VSMCs后,将其与PLGA(膜材和三维材料)进行三维培养,通过倒置显微镜、扫描电镜和MTT法观察细胞的生长情况.(2)采用全骨髓培养法体外培养rBMSCs(设实验组及对照组).应用肝细胞生长因子(HGF)诱导分化,采用倒置相差显微镜观察细胞形态变化,ELISA等方法检测甲胎蛋白,白(清)蛋白及碱性磷酸酶.结果 (1)体外培养的人胚VSMCs生长旺盛,与PLGA具有良好的相容性,在PLGA上生长良好.(2)rBMSCs经体外培养诱导后呈多角形、卵圆形改变,白(清)蛋白,AFP表达阳性,碱性磷酸酶出现明显改变.结论 (1)体外培养的人胚VSMCs成功培养纯化并与PLGA有良好的相容性,(2)在HGF诱导下,rBMSCs体外具有向肝干细胞分化的能力,为进一步分化为胆管内皮细胞提供实验依据.
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