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Alteration of the heterogeneity of Na+ channel in rabbit ventricular myocytes under simulated Ischemia
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.11.005
- Key Word:
- 心肌缺血/病理生理学;心室;心肌;钙通道
Abstract： 目的 探讨缺血时心肌细胞钠通道的异质性变化.方法 以酶解法分离兔心室肌三层细胞,先后用正常和缺血液灌流模拟正常和缺血状态,并采用膜片钳全细胞记录法观察三层心室肌细胞快钠通道的活性和异质性变化.结果 缺血后三层细胞失活曲线均左移,失活加速,三层细胞间INa失活半电压差异发生变化;缺血30 min时INa灭活后恢复曲线与原来的三层细胞间由差异无统计学意义(P＞0.05)变为差异有统计学意义(P＜0.05).各层细胞自身INa灭活后恢复随着缺血时间延长而减慢,但无统计学意义;缺血后三层细胞的钠电流(INa)电压依赖性、I-V曲线的形态轨迹未变,INa峰电流密度减小,三层细胞间峰电流密度差异发生变化.结论 缺血时心肌钠通道的活性发生变化,从而影响心室肌三层细胞间INa的异质性及与通道电流平衡,与缺血性心律失常的发生有关,并可部分解释抗心律失常药物在正常和缺血心肌的不同作用.
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