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Differentiation of fetal mesenchymal stem cells into pancreatic islet -like clusters in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.10.001
- Key Word:
- 间质干细胞;骨髓细胞;胰岛
Abstract： 目的 探索体外诱导胎儿骨髓间充质干细胞(MSCs)分化为胰岛样细胞的最佳分化条件.方法 取流产胎儿的长骨骨髓,体外培养扩增MSCs,先后予以2-巯基乙醇、表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)、B27及尼克酰胺,通过三阶段诱导MSCs分化为胰岛样细胞,观察细胞形态变化、免疫荧光鉴定巢素蛋白(nestin)、胰十二指肠同源异型盒基因(PDX-1)、胰岛素(insulin)和胰高糖素(glucagon)的表达、双硫腙染色鉴定胰岛细胞中的锌离子、电化学发光法测定人胰岛素的分泌量.结果 MSCs易于体外分离和扩增,诱导二阶段细胞表达nestin及PDX-1,诱导三阶段形成胰岛样细胞团,表达insulin及glucagon;双硫腙染色呈棕红色;胞浆胰岛素分泌量为81.3±23.6 μu /ml;有一定程度葡萄糖刺激的胰岛素释放.结论 MSCs可以诱导分化为胰岛样细胞团,尼克酰胺20 mmol/L是合适的促进胰岛细胞分化和成熟的浓度.
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