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Diagnostic value of DNA probes in the detection of pathogenic bacteria of ventilator-associated pneumonia
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.08.003
- Key Word:
- 肺炎/病因学/微生物学;通气机,机械/副作用;DNA探针
Abstract： 目的建立一种早期、快速检测呼吸机相关性肺炎致病菌的分子生物学方法.方法采用聚合酶链反应合成铜绿假单胞菌、甲氧西林耐药金黄色葡萄球菌、大肠杆菌、肺炎克雷伯杆菌和流感嗜血杆菌这5种细菌的特异DNA探针和细菌16SrRNA的通用探针,分别与生物素标记的细菌、病毒和真菌DNA杂交.杂交法和培养法同时检测100份痰液标本.结果所合成的DNA探针具有高度特异性,与其他细菌、病毒、真菌间无交叉反应.该方法可检测出1 ng细菌DNA.杂交法阳性率显著高于培养法.结论所合成的DNA探针敏感性高,特异性强,具有较高的应用价值,可应用于临床标本检测.
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