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Serologic proteome analysis of human lung adenocarcinoma
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.08.001
- Key Word:
- 肺肿瘤/病理学;腺癌/病理学;蛋白质组学;电泳,凝胶,双向;光谱法,质量,基质辅助激光解吸电离
Abstract： 目的采用以肿瘤免疫学与蛋白质组学研究技术有机地结合为基础的血清蛋白质组学研究体系(SERPA)筛选肺腺癌相关抗原.方法应用SERPA对4例人肺腺癌组织进行研究,分析肺腺癌组织总蛋白分别与肺腺癌患者的自身血清以及正常对照血清反应的Western blot图谱,识别鉴定差异反应的蛋白质.结果获得了分辨率较高的人肺腺癌组织与患者的自身血清以及正常对照血清的Western blot图谱, 共识别27±5个差异反应的蛋白质.对其中的部分差异蛋白质点进行了肽质指纹图分析,鉴定出一些与细胞生长增殖、细胞代谢、细胞周期调控、信号转导等有关的肺腺癌相关抗原.结论本研究为进一步筛选用于肺腺癌诊断、治疗和预后评估的肺腺癌分子标志物奠定了坚实的基础.
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