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The location of the arachnoid granulations in cranial cavity and the apoptosis after subarachnoid hemorrhage in rats
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.07.001
- Key Word:
- 蛛网膜下腔出血;蛛网膜/超微结构;脑/超微结构
Abstract： 目的研究大鼠蛛网膜颗粒在颅内的分布,为实验取材及其相关研究奠定基础.通过动物实验分析蛛网膜下腔出血(SAH)后的蛛网膜颗粒细胞凋亡.方法亚甲蓝组大鼠小脑延髓池内注入亚甲蓝,亚甲蓝广泛分布于蛛网膜下腔,蛛网膜颗粒摄取亚甲蓝呈现兰色,记数蛛网膜颗粒的数目及分布.SAH组分为A、B、C、D、E组,A组为对照组,小脑延髓池内注入生理盐水,B、C、D、E组为实验组,小脑延髓池注入自体动脉血溶解物,于10 d处死对照组(A组)大鼠,分别于注血后1 d(B组)、4 d(C组)、7 d(D组)、10 d(E组)处死实验组大鼠.取硬脑膜窦进行石蜡包埋、切片,采用TUNEL法检测蛛网膜颗粒的细胞凋亡.结果 90.7%的蛛网膜颗粒分布于横窦内,6.4%分布于矢状窦内,2.9%分布于硬脑膜.A组蛛网膜颗粒中偶见细胞凋亡, B、C、D、E 4组蛛网膜颗粒中凋亡细胞逐渐增多.蛛网膜细胞、内皮细胞及纤维细胞三类细胞均有凋亡现象.结论大鼠的蛛网膜颗粒主要分布在横窦内.SAH后存在过度的炎症反应过程,使蛛网膜颗粒出现过度的细胞凋亡.
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