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Experimental study on oligopeptide K16GRGDSPC as a targeted vector for gene transfection
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.04.001
- Key Word:
- 基因转移技术;骨髓祖代细胞;甘氨酸;精氨酸;丝氨酸;天冬氨酸;赖氨酸;脯氨酸;半胱氨酸
Abstract： 目的构建一种新型靶向性非病毒基因转移载体-含RGD肽K16GRGDSPC(记作K16-RGD),评价其介导外源基因转染骨髓基质干细胞的可行性和靶向特异性.方法固相合成法合成K16-RGD,以其为载体将虫荧光素酶质粒导入兔骨髓基质干细胞,检测其转染效率;并观察溶酶体抑制剂氯喹和聚乙烯亚胺(PEI)对其转染效率的影响.通过细胞黏附竞争性抑制和转染竞争性抑制实验检测K16-RGD介导转染的靶向特异性.结果 K16-RGD的转染效率低于商业化脂质体,但加入氯喹可使其转染效率与脂质体相近.PEI也能显著提高K16-RGD的转染效率.含RGD肽GRGDSPC、K16GRGDSPC能显著抑制BMSCs的黏附和降低K16-RGD的转染效率.结论 K16-RGD是一种易于合成、高效、低毒的新型靶向性非病毒基因转移载体,以其作为载体介导外源基因转染骨髓基质干细胞是可行的,为下一步构建载基因仿生基质材料进行骨缺损修复研究奠定了基础.
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