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The distinguishing of gastric cancer associated genes by digital gene expression displayer using serial analysis of gene expression database
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.03.005
- Key Word:
- 胃肿瘤;基因组;基因组学;自动数据处理
Abstract： 目的对肿瘤基因组解剖计划获得的海量数据进行充分数据挖掘,筛选胃癌差异表达基因.方法采用数字化基因差异显示技术对肿瘤基因组解剖计划数据库中的4个胃癌和2个正常胃组织文库共300,783条基因表达系列分析数据进行了分析.结果筛选出差异表达的已知基因136个,其中胃癌中表达上调的有54个,下调82个,EST序列65条,其中上调24条,下调41,虚拟Northern和数字化显示对筛选出的差异表达基因PTMA构建了全身正常组织和肿瘤组织的基因表达图谱.结论充分利用生物信息学工具可以快速有效地筛选肿瘤差异表达基因,指导后续分子生物学实验研究.筛选出来的差异表达基因通过验证,有望成为新的胃癌分子靶标.
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