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The effects of all-trans-retinoic acid on the proliferation and apoptosis of smooth muscle cells in human saphenous vein organ culture
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.02.005
- Key Word:
- 维甲酸/药理学;细胞凋亡/药物作用;静脉/移植;肌,平滑,血管/药物作用/细胞学
Abstract： 目的研究全反式视黄酸(atRA)对体外培养的人体大隐静脉内膜增厚、平滑肌细胞增生与凋亡的影响.方法30小段(1 cm长)人体大隐静脉(HSV),随机分成HSV组、atRA组和对照组(n=10),atRA组和对照组均体外培养14 d.atRA组:培养液中含atRA100μM;对照组:培养液中含同等量无水酒精(atRA溶剂).对HSV组及培养后的atRA组、对照组标本进行取材固定,Masson染色,免疫组化测定增殖细胞核抗原(PCNA)及TUNEL法检测平滑肌细胞凋亡,计算机图象分析仪测量血管内膜厚度、计算内膜增生指数及细胞增殖和凋亡百分比.结果体外培养人体大隐静脉能产生明显的内膜增生和平滑肌细胞增殖(P＜0.05);atRA组内膜厚度、增生指数及PCNA阳性细胞百分数均明显低于对照组(P＜0.05).而凋亡细胞百分数则明显高于对照组(P＜0.05).结论atRA可以抑制体外培养人体大隐静脉内膜增厚及平滑肌细胞增生,促进平滑肌细胞凋亡.
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