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The cardioprotective effect of the inhibitor of JAK/STAT on simulating ischemia-reperfusion injury in cultured immature rat cardiacmyocytes
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-1372.2006.02.001
- Key Word:
- 心肌再灌注损伤;转录启动子;信号传导
Abstract： 目的研究抑制Janus激酶/信号转导和转录激活子(JAK/STAT)通路对乳鼠心肌细胞缺血再灌注损伤的保护作用.方法采用体外培养的新生大鼠心肌细胞造成缺血再灌注(I/R)模型,随机分为正常对照组,缺血再灌注组,JAK抑制剂-AG490治疗组,STAT抑制剂-RMP治疗组.采用MTr法检测心肌细胞活性,检测以上不同条件下细胞上清液中的乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量,并检测各组细胞的凋亡率.结果与正常对照组相比,缺血再灌组心肌细胞成活率明显降低(P＜0.01),细胞上清液中LDH、MDA含量明显升高(P＜0.01),SOD活力则显著降低(P＜0.01),缺血再灌组凋亡率升高明显(P＜0.01).AG490及RMP处理后,心肌细胞成活率明显升高(P＜0.01),LDH、MDA显著低于缺血再灌组(P＜0.01),SOD则高于缺血再灌组(P＜0.01).结论抑制JAK/STAT通路有助于减轻缺血再灌注所致心肌损伤.
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