You Position: Home > Paper
Expressions of p63 mRNA and murine double minute 2 in esophageal cancer and molecular mechanisms involved
( views:169, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.11.004
- Key Word:
- 食管癌;p63;MDM-2
Abstract： 目的探讨p63mRNA、MDM-2蛋白在食管鳞癌中的表达及其在食管鳞癌形成和预后中的意义.方法采用RT-PCR和免疫组化技术分别检测38例食管鳞癌组织及其对应的正常组织中p63mRNA和MDM-2蛋白的表达水平.结果ΔNp63mRNA在食管鳞癌组织和正常组织中阳性检出率分别为63.15%和52.63%,二者比较无显著性差异;其中低分化食管鳞癌组ΔNp63mRNA的阳性表达率显著高于高/中分化食管鳞癌组,而与年龄、性别、淋巴结转移和临床分期无关.38例食管鳞癌组织中仅有1例呈TAp63 mRNA阳性表达,正常组织样品中未检出有TAp63 mRNA阳性表达.MDM-2蛋白在食管鳞癌组织中的阳性表达率为65.79%,显著高于正常组织,低分化食管鳞癌组MDM-2蛋白的阳性表达率显著高于高/中分化组,而且随临床分期的程度增高而阳性表达率升高,而与年龄、性别、淋巴结转移无关.随着MDM-2蛋白表达率的增加,ΔNp63mRNA的表达率相应增加,二者的表达呈正相关.结论 p63基因在食管鳞癌和食管上皮中表达的亚型主要是ΔNp63,ΔNp63参与了食管鳞癌的发生、发展,并与食管鳞癌的预后相关;TAp63不是食管鳞癌和食管上皮中主要的表达亚型,与食管鳞癌的形成基本无关;MDM-2蛋白的表达异常在食管鳞癌形成中起重要作用,而且与鳞癌的预后相关;ΔNp63通过MDM-2发挥其对细胞周期及凋亡的调节作用,进而导致食管鳞癌的形成是其主要的分子机制.
- [1]Tokino T,Morimoto I,Imai K,etc.Adenovirus-mediated transfer of the p53 family genes, p73 and p51/p63 induces cell cycle arrest and apoptosis in colorectal cancer cell lines: potential application to gene therapy of colorectal cancer.[J].2001,8(18).
- [2]Klimpfinger M,Kohlhaw K,Schmelzer S,etc.Expression of the p53 homologues p63 and p73 in multiple simultaneous gastric cancer.[J].2001,195(2).
- [3]Ito, Yasuhiro,Takeda, Tsutomu,Wakasa, Ken-Ichi,etc.Expression of p73 and p63 proteins in pancreatic adenocarcinoma: p73 overexpression is inversely correlated with biological aggressiveness[J].2001,8(1).