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Detection of lymph node micrometastasis of gastric cancer using monoclonal anti-cytokeratin (AE1/AE3) antibody immunohistochemistry
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.11.003
- Key Word:
- AE1/AE3;胃癌;淋巴结;微转移
Abstract： 目的探讨AE1/AE3免疫组化标记检测胃癌根治标本中淋巴结内微小转移癌及其临床意义.方法对64例胃癌根治切除后阴性的淋巴结及胃周软组织的石蜡包埋组织用S-P法进行AE1/AE3染色标记.结果 64例118枚淋巴结中,根据HE染色阴性再经AE1/AE3标记发现,8例患者13枚淋巴结发现微转移病灶,病例总阳性率12.5%(8/64),淋巴结总阳性率11.02%(13/118).微转移与浸润深度有关,深层组织的微转移阳性率高于浅层(P＜0.05),弥漫型胃癌微转移率(62.50%)明显高于肠型胃癌(5.36%),两者之间具有显著差异性.结论胃癌周围阴性淋巴结中存在微转移灶,AE1/AE3染色标记检测微转移淋巴结可能对准确地确定临床分期、指导治疗、判断预后有积极临床意义.
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