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Effects of loading trehalose into cytoplasm before freeze-drying on platelets activation and response to agonists in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.11.001
- Key Word:
- 海藻糖;血小板激活;聚集;激活抑制剂
Abstract： 目的研究血小板在温度37℃条件下, 负载海藻糖4h后,其体外激活程度和聚集反应性的变化.方法分别以凝血酶、ADP、胶原、瑞斯托霉素4种物质作为血小板激活诱导剂,用APACT2型血小板聚集仪分别检测血小板负载海藻糖前后的聚集反应性.用流式细胞术检测分析血小板负载海藻糖前后其膜表面糖蛋白分子CD62p、PAC-1的表达率,加可逆性激活抑制剂PGE-1、腺苷后血小板激活被抑制的程度.结果同负载前相比较,血小板平均体积(MPV)、血小板对四种激活诱导剂的最大聚集率均无显著性差别(p＞0.01),经4h负载海藻糖后,血小板膜表面CD62p表达率升高,但联合添加可逆性激活抑制剂PGE-1、腺苷后,CD62p表达率显著下降,PAC-1表达率始终维持恒定.结论加可逆性激活抑制剂后,冻干前预处理血小板过程对血小板的功能活性没有显著影响.
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