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Application of Gene Chip for Detecting Mutations of Rifampin-resistant Genes in Mycobacterium Tuberculosis Strains
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.09.003
- Key Word:
- DNA芯片;rpoB基因;利福平;耐药性;结核分枝杆菌
Abstract： 目的研制一种新型DNA芯片,用于快速检测结核分枝杆菌耐利福平rpoB基因突变.方法根据结核分枝杆菌rpoB基因序列设计探针并制作DNA芯片,PCR掺入法荧光标记根据结核分枝杆菌rpoB基因突变热点的目的片断,与DNA芯片杂交,同时以DNA测序法为对照.结果 18株结核分枝杆菌临床分离株中,1株敏感株DNA芯片杂交结果与标准株完全相同;17株耐RFP临床分离株中有17株检测到rpoB基因突变,其中14株单位点突变,1株511和516位双位点突变,与测序结果完全一致.另有两株为517和518、526和517位双位点突变,因为芯片上未点517位突变的探针,所以只检测到518和526位的突变,该突变与测序结果完全一致.结论用DNA芯片可快速、特异地检测出大多数结核分枝杆菌耐利福平分离株的rpoB基因突变,可用于临床耐药性的检测,指导临床用药.
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