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Construction of Antisense GA cDNA Recombinant Plasmid and Its Effect on the Growth of Colon Cancer Cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.08.001
- Key Word:
- 基因重组;质粒;结肠癌;增殖
Abstract： 目的构建含GA反义基因的重组质粒,研究GA反义基因对结肠癌细胞生物学性状的影响,为利用GA反义基因治疗肿瘤提供依据.方法应用基因重组技术构建含GA反义基因的重组质粒pcDNA3.0/GA,通过脂质体法转染人结肠癌细胞DH5α,MTT法、电镜观察等方法检测GA反义基因对结肠癌细胞生物学性状的影响.结果获得含GA反义基因的pcDNA3.0/GA,转染结肠癌细胞DH5α后,结肠癌细胞生长速度明显减慢,细胞超微结构呈典型的凋亡改变.结论 GA反义基因可抑制肿瘤细胞内GA基因的表达,使细胞内GA活性下降,并导致肿瘤细胞利用Gln障碍,最终使肿瘤细胞生长受抑制.
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