You Position: Home > Paper
The Effect of Fas and FasL Abnormal Expression on Immune Escape Mechanism of Renal Cell Carcinoma
( views:310, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.06.002
- Key Word:
- Fas;FasL;凋亡;免疫逃避;肾细胞癌
Abstract： 目的探讨肾癌系统表达异常对其免疫逃避机制的影响.方法采用免疫组化方法分析44例肾癌组织及相邻正常肾组织Fas、FasL表达情况;用TUNEL法检测44例肾癌标本中浸润性T淋巴细胞的凋亡.体外培养786-0肾癌细胞株,流式细胞仪（FCM）分析肾癌细胞株Fas、FasL的表达及肿瘤细胞的凋亡;体外培养Fas+的Jurkat细胞,FCM检测其凋亡情况.结果肾癌细胞Fas表达率为22.8%,显著低于其在正常肾组织中表达率（53.8%,P＜0.01）.而FasL表达率（46.5%）显著高于其在相邻正常肾组织在的表达（23.2%）.肾癌组织Fas、FasL表达呈负相关（r=-0.46,P＜0.05）;肾癌浸润性T淋巴细胞凋亡率（33.1%）与肾癌FasL表达呈正相关（r=0.96,P＜0.01）.化疗药物5-Fu能上调肾癌细胞株786-0Fas的表达,并增强肾癌细胞对Fas抗体的细胞毒效应,通过抑制肾癌细胞株786-0FasL表达而降低其攻击Jurkat T淋巴细胞的能力.讨论 肾癌细胞免疫逃避机制包括两方面：一方面通过Fas低表达,能逃避Fas单克隆抗体介导的凋亡;另一方面通过FasL高表达,具有主动攻击免疫淋巴细胞的能力.临床化疗药物5-Fu就Fas系统而言通过上调Fas、抑制FasL的表达发挥一定的疗效.
- [1]姜福金,郑骏年,孙晓青,等.肾癌FasL表达异常对T淋巴细胞凋亡的影响[J].2001,(5).
- [2]Arai K,Yoshida K,Kamai T,etc.Fas labeling status does not correlate with apoptosis of renal cell carcinoma in vivo.[J].2001,21(5).
- [3]M, Weller,U, Malipiero,A, Rensing-Ehl,etc.Fas/APO-1 gene transfer for human malignant glioma.[J].1995,55.
- [4]L, Peduto Eberl,L, Guillou,E, Saraga,etc.Fas and Fas ligand expression in tumor cells and in vascular smooth-muscle cells of colonic and renal carcinomas.[J].1999,81.
- [5]陈智,刘克洲,章明太,等.肝细胞癌患者癌及癌旁组织中FAS及配体的定位[J].1997,(3).
- [6]Elsasser Beile U,Gierschner D,Welchner T,etc.Different expression of Fas and Fas ligand in tumor infiltrating and peripheral lymphocytes of patients with renal cell carcinomas.[J].2003,23(1A).
- [7]Sejima T,Isoyama T,Miyagawa I.Alteration of apoptotic regulatory molecules expression during carcinogenesis and tumor progression of renal cell carcinoma.[J].2003,10(9).
- [8]Elizabeth G. Nabel,Hiroshi Arai,avid Gordon.Gene transfer of Fas ligand induces tumor regression in vivo[J].1997,94(25).