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Experimental Study on Radioimmunoimaging of Anti-human PSMA Monoclonal Antibody Labeled with 131 I in Nude Mice Bearing Prostate Cancer
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.06.001
- Key Word:
- 前列腺特异膜抗原;单克隆抗体;前列腺肿瘤;放射免疫显像
Abstract： 目的研究131I标记的抗前列腺特异膜抗原(PSMA)单克隆抗体(131I-Ed-5))在荷人前列腺癌瘤裸鼠体内的分布及对肿瘤的靶向定位性能.方法建立荷人前列腺癌裸鼠移植瘤模型,用131I标记抗PSMA单克隆抗体Ed-5,131I-Ed-5经尾静脉注入荷瘤裸鼠体内,于注射后24、48、96、144hγ照相后断颈处死,取脏器称湿重并计数,测定肿瘤和血液、肝、肺等重要脏器的单位重量放射性比值(T/NT)、各组织摄取百分比(%ID/g).结果在注射131I-Ed-5后96h,肿瘤显影清晰,随时间延长其边缘更清晰.在注射131I-Ed-5后24～144h内,裸鼠体内肿瘤部位出现选择性放射性浓聚;96h肿瘤对131I-Ed-5的摄取率为32.30%ID/g,各组织的T/NT比值也达到最高,其中肿瘤/肌肉高达7.08.给予注射131I-NMIgG的对照组中,未出现放射性浓聚,而呈全身分布.Ed-5具有良好的免疫活性和对前列腺癌的靶向定位作用,可望用于前列腺癌导向诊断与放射免疫治疗.
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