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Role of Apoptosis in the Gut Mucosal Barrier Dysfunction in Rats with Cardiopulmonary Bypass
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.03.004
- Key Word:
- 体外循环;细胞凋亡;肠黏膜屏障;大鼠
Abstract： 目的探讨细胞凋亡在大鼠体外循环(CPB)早期肠道黏膜屏障功能障碍中的作用.方法建立大鼠CPB模型.分别对CPB组、假手术组(SO)和正常组(N)大鼠于术后3、6、12、24h取回肠末端黏膜进行光镜、电镜观察,并用TUNEL方法研究肠上皮细胞凋亡.结果 CPB组大鼠除了24h时相点光镜下观察发现肠黏膜上皮细胞脱落外,其它时相点肠粘模均保持完整,电镜观察可见CPB各时点均可见典型凋亡细胞;TUNEL法显示CPB组各时相点肠黏膜上皮细胞凋亡指数较SO组显著增加(P值＜0.01),并于CPB结束后6h达到峰值,凋亡细胞主要分布在肠黏膜隐窝部.结论大鼠CPB后早期肠黏膜上皮细胞凋亡明显增加,上皮细胞凋亡增加可能是CPB后肠黏膜屏障功能障碍的病理生理基础.
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