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The Change of CD4+CD25+ and CD8+CD28- T Regulatory Cells in Rats with the Experimental Colitis Induced by TNBS
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.02.005
- Key Word:
- TNBS;实验性结肠炎;调节性T细胞
Abstract： 目的探讨CD4+CD25+和CD8+CD28-调节性T细胞在TNBS(2,4,6-三硝基苯磺酸)诱发的大鼠实验性结肠炎的外周血、脾脏、结肠的改变及其作用.方法建立TNBS实验性结肠炎大鼠模型, 设对照组、建模后第一周和第三周三组,用流式细胞仪检测各组外周血、脾脏和结肠黏膜上皮细胞内单个核细胞中CD4+CD25+和CD8+CD28-调节性T细胞比例的变化.结果成功建立TNBS大鼠实验性结肠炎模型(n=15).结肠部位CD8+CD28-细胞在建模后第一周时较对照组均明显增高[(12.7±5.4)% vs(3.87±3.7)%,P＜0.01].在外周血、脾和结肠中CD8+CD28-T细胞比例在建模第三周时均较对照组明显增加[外周血:(14.4±5.5)% vs(6.5±4.6)% ,脾脏:(18.7±4.9)% vs(7.2±5.7)%,结肠:(16.3±4.9)% vs(3.87±3.7)%,P＜0.01],其中以结肠部位增加最高,同其它两部位比较有统计学差异(2.8±0.6 vs 1.1±0.4,2.8±0.6 vs 1.5±0.4,P＜0.05).在第一周,CD4+CD25+T细胞在结肠部位较对照组增加[(13.6±6.1)vs(5.7±4.2),P＜0.05],于第三周回落至对照组水平.结论 CD4+CD25+和CD8+CD28-T细胞在TNBS实验性结肠炎大鼠模型中较正常对照组有明显增加,但增加的部位和时间点有所不同,提示这两种调节性T细胞可能在溃疡性结肠炎发病的不同阶段中起着一定作用,且主要作用于肠道病变部位.
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