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Construction of Retroviral Recombinant Vectors Carrying Sense-and Antisense-hGRα cDNA by an Improved Ligasing Protocol
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1008-1372.2005.01.003
- Key Word:
- 人糖皮质激素受体α;连接;反义RNA
Abstract： 目的构建人糖皮质激素α(hGRα)cDNA的表达重组体,为较大DNA片段的克隆提供借鉴,并为利用反义核酸技术探讨hGRα功能及其作用机制提供实验材料.方法 L-PCR扩增hGRα cDNA,克隆至T载体;随后通过改进的连接方案进行亚克隆.结果限制性酶增和序列分析表明克隆及亚克隆成功.结论成功构建于hGRα cDNA的逆转录病毒载体pLXSN正义及反义重组子.
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