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DNA Microarray Probe Preparation Using cDNA Fragments Library from HCV Genotype 1
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1008-1372.2004.06.003
- Key Word:
- HCV;分子克隆;microarray;分子探针
Abstract： 目的探讨利用cDNA文库法制备丙型肝炎(HCV-1)诊断基因芯片探针的可行性.方法用限制性内切酶Sau3AⅠ消化HCV 1 a及1b全长cDNA,所得的酶切片段72℃补平加A,AT克隆,PCR初步鉴定,并测序.结果 HCV两个亚型1a、1b的全长cDNA得到57个大小相对一致(200-1000bp)的片段,平均每个亚型约28个,PCR及序列分析表明,所扩增的片段均属于HCV-1的特异基因,可做为HCV-1诊断基因芯片探针.结论利用cDNA文库法收集片段是一种快速、简便制备芯片探针的实用方法.
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