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Differential Analysis of Two-dimensional Gel Electrophoresis Profiles of Gastric Carcinoma and Paired Normal Gastric Mucosa
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1008-1372.2004.04.004
- Key Word:
- 胃癌;双向凝胶电泳;蛋白质组
Abstract： 目的利用蛋白质组学方法建立分辨率高和重复性好的人胃癌组织及其正常胃黏膜组织的双向凝胶电泳图谱,分析其差异表达的蛋白质,为进一步寻找胃癌标志物奠定基础.方法采用固相pH梯度双向凝胶电泳分离人胃癌组织及正常胃黏膜组织的总蛋白质,凝胶经银染显色后,Imaging Master 2D图像分析软件进行比较分析,识别差异表达的蛋白质.结果(1)癌组织和正常胃黏膜组织凝胶的平均蛋白质点数分别为1049±67和1097±73,平均匹配的点数分别为835±48和953±56,匹配率分别为79.6%和86.7%.(2)通过比较分析5例胃癌组织及正常胃黏膜组织的双向凝胶电泳图谱,得到平均匹配蛋白点数为769±45,差异表达蛋白点数为81个,其中17个点仅在癌组织中表达,24个点仅在正常胃黏膜中表达,26个点在癌组织中为高表达,14个点在癌组织中为低表达.结论本研究建立了分辨率高且重复性较好的人胃癌组织与正常胃黏膜组织的双向凝胶电泳图谱,发现两者间存在一些差异表达的蛋白质,为进一步筛选胃腺癌特异性的分子标志物打下了坚实的基础.
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