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The Effect of Mesenchymal Stem Cells Transplantation on Heart Function of Rat Myocardial Infarction
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1008-1372.2004.04.002
- Key Word:
- 间充质干细胞;移植;心肌梗死;心功能;钙泵
Abstract： 目的探讨骨髓间充质干细胞心肌移植对大鼠心梗后心功能及钙泵的影响.方法取大鼠骨髓间充质干细胞进行体外培养、增殖和标记,同时以液氮冷冻左室游离壁建立心梗模型.4周后分别将2×106个细胞悬液或冷D-Hanks液注入实验组和对照组心梗区数个不同部位.另取5只大鼠作为正常组,仅行两次开胸手术.4周后行心功能测定及取标本,RT-PCR检测SERCA2基因mRNA的表达.结果骨髓间充质干细胞于移植后1周、2周和4周后均可存活并具有分化成熟倾向.Buxco系统行有创动态心功能测定,发现SLVP、+dp/dt以正常组为最高,实验组次之,对照组最低.而LVEDP则相反以正常组最低,实验组次之,对照组为最高.心梗后SERCA2基因mRNA表达水平对照组较正常组明显降低(0.988±0.004对1.824±0.009,P＜0.01).骨髓间充质干细胞心肌移植后,虽然SERCA2基因mRNA表达水平实验组也较正常组有所降低(1.583±0.012对1.824±0.009,P＜0.01),但与对照组相比明显升高(1.583±0.012对0.988±0.004,P＜0.01).结论骨髓间充质干细胞心肌移植可促进SERCA2mRNA表达,并使大鼠心梗后受损的心功能改善.
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