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Expression of p53 Protein of Hepatocellular Carcinoma Cells after Transcatheter Arterial Chemoembolization
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2004.03.006
- Key Word:
- 肝细胞癌;经动脉化疗栓塞;细胞凋亡;细胞增殖;p53
Abstract： 目的评价肝细胞癌p53蛋白表达在经导管动脉化疗栓塞(transcatheter arterial chemoembolization, TACE)中的作用.方法经手术病理证实的肝细胞癌136例,其中行1～5次TACE后Ⅱ期手术切除79例(TACE组),按治疗方式不同分4组,A组,仅灌注化疗药物11例,治疗1～4次;B组,化疗药加碘化油栓塞33例,治疗1～5次;C组,化疗药加碘化油加明胶海绵颗粒栓塞23例,治疗1～3次;D组,化疗药加碘化油、无水乙醇、明胶海绵颗粒栓塞12例,治疗1～3次.单纯手术57例(非TACE组).用TUNEL法检测AI,用免疫组化检测各标本Bcl-2, Bax, p53, Ki-67和PCNA蛋白表达.结果梁索型、透明细胞型p53蛋白表达显著低于假腺样型、实体型、低分化或未分化型、硬化型(P＜0.05);随着病理分级的增高,p53蛋白表达也逐渐增多(P＜0.05);p53蛋白表达与PCNA和Ki-67蛋白表达显著正相关(P＜0.05),与AI,Bcl-2/Bax蛋白表达率显著负相关(P＜0.05).p53蛋白表达A组显著高于B、C、D组和单纯手术组;B组显著高于C和D组;D组显著低于单纯手术组(P＜0.05).结论在肝细胞癌化疗栓塞中p53蛋白表达起增强癌细胞增殖、抑制细胞凋亡的作用.
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