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The Influence of Composite Transplantation of Two Kinds of Acellular Xeno-dermis and Micro-autograft on Wound Healing
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2004.03.002
- Key Word:
- 微粒皮;选择性去细胞异种(猪)皮;复合皮移植
Abstract： 目的研究自体微粒皮与异种(猪)去细胞真皮基质及选择性去细胞异种(猪)皮复合移植对创面愈合的影响.方法以72只SD大鼠为动物模型分为复合皮组I、复合皮组II和微粒皮组,复合皮组I于背部全层皮肤缺损创面移植异种(猪)无细胞真皮+自体微粒皮+选择性去细胞异种(猪)皮,复合皮组II移植异种(猪)无细胞真皮+自体微粒皮+戊二醛异种(猪)皮,微粒皮组移植自体微粒皮.术后定期观察创面愈合情况,计算创面收缩率和创面愈合率,取活检标本行组织学、免疫组化及电镜观察.结果经组织学,免疫组化染色及电镜观察,选择性去细胞异种(猪)皮表皮层连续、完整,真皮中三大纤维排列有序且无细胞成分,基底膜结构完整;复合皮组I上皮化良好,胶原纤维排列有序,免疫组化染色证实有完整的基底膜生成.复合皮组I的创面愈合效果优于复合皮组II及微粒皮组.结论选择性去细胞异种(猪)复合皮(异种无细胞真皮+自体微粒皮+选择性去细胞异种皮)可作为一种新型的皮肤替代物修复全层皮肤缺损.
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