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Effect of Nao Yi An on VEGF Expression in Rat Brain with Cerebral Hemorrhage
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-1372.2004.02.004
- Key Word:
- 血管内皮生长因子;脑出血;免疫组织化学;原位杂交;脑溢安
Abstract： 目的研究实验性脑出血大鼠脑内血管内皮生长因子(VEGF)的时空分布及中药脑溢安的影响.方法通过立体定位向脑内苍白球注入Ⅶ型胶原酶0.4U建立脑出血模型,运用免疫组化和原位杂交法检测脑出血后3h、6h、12h、1d、4d、7d,14d七个时间点VEGF的表达变化,以阳性细胞数作为观察指标.结果脑出血3hVEGF开始表达,6h增强,12h及1d阳性细胞达到高峰,4d开始下降,到7d和14d仍有少量表达.脑溢安组在6h、12h、1d、4d高于模型组(P＜0.05).结论脑出血后脑内VEGF表达增加;脑溢安可上调脑出血后VEGF 的表达,可能是其神经保护机制之一.
- [1]罗杰坤,黎杏群,张花先.脑溢安对大鼠脑出血后Caspase-3表达的影响[J].2002,(2).
- [2]Sun Y,Xie L,Childs J,etc.VEGF-induced neuroprotection, neurogenesis, and angiogenesis after focal cerebral ischemia.[J].2003,111(12).
- [3]Rao S,Goldman SA,Leventhal C,etc.Coordinated interaction of neurogenesis and angiogenesis in the adult songbird brain.[J].2002,34(6).
- [4]Levkau B,Arolt V,Peters M,etc.Vascular Endothelial Growth Factor Protects Cultured Rat Hippocampal Neurons Against Hypoxic Injury via an Antiexcitotoxic, Caspase-Independent Mechanism.[J].2002,22(10).
- [5]Zhu Y,Greenberg DA,Jin K,etc.Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo.[J].2002,99(18).
- [6]Goldsmith PC,Greenberg DA,Jin KL,etc.Induction of vascular endothelial growth factor receptors and phosphatidylinositol 3'-kinase/Akt signaling by global cerebral ischemia in the rat.[J].2000,100(4).
- [7]Jin KL,Greenberg DA,Mao XO.Vascular endothelial growth factor: direct neuroprotective effect in in vitro ischemia.[J].2000,97(18).