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Experimental Study on the Preventive Mechanism of Anisodamine on Multiple Organ Dysfunction Syndrome
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1008-1372.2004.01.003
- Key Word:
- 山莨菪碱;多器官功能障碍综合征;I-κB激酶(IKK-β);核因子-κB;肿瘤坏死因子-α
Abstract： 目的探讨山莨菪碱(654-2)对多器官功能障碍综合征(MODS)可能的治疗机制.方法采用失血性休克合并内毒素刺激诱发MODS家兔模型,24只大耳白兔随机分为正常对照组(C组)、模型组(M组)、654-2治疗组(T组).用原位杂交(ISN)技术、凝胶电泳迁移率改变(EMSA)及酶联免疫吸附试验(ELISA)法分别检测肺泡巨噬细胞(PAM)及肝枯否细胞(KC)中I-кB激酶(IKK-β)mRNA表达、NF-кB的活性和细胞培养上清液中TNF-α的含量.并进行动脉血气、生化和内脏组织病理学光镜检查.结果 M组和C组比较PAM和KC中IKK-β mRNA表达[(0.15±0.03 );(0.17±0.04 )],NF-кB活性[(1.49±0.30 );(1.72±0.36 )] 和上清液TNF-α的含量[( 279.74±25.91 );(300.05±30.86 )ng/L] 明显增高(P均＜0.01).肝肺肾小肠等脏器功能发生不同程度损害;654-2治疗组能显著抑制M组PAM和KC中IKK-β mRNA表达[(0.10±0.02);(0.11±0.02)]、NF-кB活性[(0.65±0.10);(0.88±0.20)]和TNF-α[(180.61±13.01);(210.28±18.01)ng/L]的分泌(P＜0.01或P＜0.05).明显缓解MODS动物内脏组织的功能损害.结论 654-2通过抑制IKK-β/NF-κB/TNF-α激活效应发挥对MODS的保护作用.
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