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The Growth-Suppressive Effect of Adenovirus-mediated Wild-type p53 Transfection on Gastric Cancer Cell Line
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1008-1372.2003.10.002
- Key Word:
- 基因治疗;p53基因;腺病毒载体;胃癌细胞
Abstract： 目的观察野生型p53基因(wtp53)对体外培养人胃癌细胞系生长的抑制作用,探讨野生型p53基因在胃癌基因治疗中的重要意义.方法以携带有野生型p53基因的复制缺陷型腺病毒(pAd-p53)为载体,转染有p53基因突变的人胃癌细胞系BGC-7901.应用生化染色,检测外源基因的转染效率;应用免疫组化、原位杂交等方法检测外源基因的表达效果;应用细胞计数、MTT法检测pAd-p53转染对胃癌细胞生长的抑制效果;应用流式细胞仪检测细胞周期.结果当腺病毒在100MOI以上效靶比转染人胃癌细胞系时,转染效率达100%.转染3d以后,胃癌细胞中突变性p53蛋白表达开始减少,野生型p53 mRNA的表达开始增多,BGC-7901细胞生长受到明显抑制,流式细胞仪检测显示G0/G1期细胞数增加(未转染组56.47%,转染pAd-p53组79.40%,P＜0.05),S期细胞数减少(未转染组30.80%,转染pAd-p53组13.81%,P＜0.01).结论腺病毒介导的野生型p53基因体外转染人胃癌细胞,可有效抑制胃癌细胞的生长,可能成为胃癌基因治疗的手段之一.
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