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Observation on Biological Character of Cultivated Cornea Limbal Epithelial Cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1008-1372.2003.04.006
- Key Word:
- 角膜缘上皮细胞;培养;生物学特性
Abstract： 目的观察体外培养的角膜缘上皮细胞生物学特性.方法体外组织块培养兔角膜缘上皮细胞,倒置显微镜观察活细胞生长情况,透射电镜观察其超微结构,用流式细胞仪检测细胞的增殖能力,使用MTT方法绘制传代细胞的生长曲线.结果角膜缘组织块培养第6d培养的角膜缘上皮细胞形成单层,培养的细胞具有代谢旺盛和较高的增殖能力,传代的角膜缘上皮细胞在第4d达生长高峰.结论体外培养的第1、2代角膜缘上皮细胞既保持角膜上皮特性,又具有强的增殖能力.
- [1]F E, Kruse,S C, Tseng.Serum differentially modulates the clonal growth and differentiation of cultured limbal and corneal epithelium.[J].1993,34(10).
- [2]薛庆善.体外培养的原理与技术[M].2002.
- [3]郑永唐,贲昆龙.测定细胞存活和增殖的MTT方法的建立[J].1992,(4).
- [4]A, Schermer,S, Galvin,T T, Sun.Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.[J].1986,103.
- [5]I R, Schwab,M, Reyes,R R, Isseroff.Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease.[J].2000,19(4).