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Inhibitory Effect of Low-Molecular-Weight Heparins on Rat Glomerulus Mesangial Cell Proliferation and MCP-1 Expression
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.1008-1372.2002.12.010
- Key Word:
- 低分子肝素;系膜细胞;单核细胞趋化蛋白-1
Abstract： 目的观察低分子肝素(LMWH)对大鼠肾小球系膜细胞(GMCs)增生及单核细胞趋化蛋白-1(MCP-1)表达的作用.方法在体外培养的大鼠GMCs株中加入脂多糖(LPS)后,再分别加入不同浓度的LMWH,设实验组1组:GMCs+LPS+LMWH,设对照组2组:GMCs+LPS组及GMCs组,分别将上述3组命名为:LMWH组,LPS组及GMCs组.采用MTT掺入方法,于24h、48h,观察3组中GMCs增生水平,并采用ELISA方法观察其培养的上清液中单核细胞趋化蛋白-1(MCP-1)的浓度.结果 LMWH组于48h GMCs增生的抑制最为显著,其中:LMWH组GMCs增生水平低于LPS组(P＜0.05),与GMCs组无统计学差别(P＞0.05);LMWH组GMCs培养上清中MCP-1浓度明显低于LPS组(P＜0.01),与GMCs组无统计学差别(P＞0.05).结论 LMWH能抑制大鼠GMCs增生,下调GMCs中MCP-1的异常表达及分泌.
- [1]M J, Koza,H L, Messmore,M E, Wallock,etc.Evaluation of a low molecular weight heparin as an anticoagulant in a model of cardiopulmonary bypass surgery.[J].1993,70(1).
- [2]陈香美,徐启河.肝素治疗肾小球疾病的机理研究进展[J].1998,(5).
- [3]S J, Shankland.Cell-cycle control and renal disease.[J].1997,52.
- [4]B H, Rovin,T, Yoshiumura,L, Tan.Cytokine-induced production of monocyte chemoattractant protein-1 by cultured human mesangial cells.[J].1992,148(7).
- [5]潘晓勤,王晓燕,姜新猷.肾小球系膜细胞培养及鉴定[J].1995,(1).
- [6]FrdrckWK am,AymanM Karkar,Jnnfr Smh,etc.Differential expression of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1 in experimental glomerulonephritis[J].1996,49.
- [7]易著文主编.小儿临床肾脏病学[M].1998.
- [8](美) F.奥斯伯… [等] 著 颜子颖,王海林译.精编分子生物学实验指南[M].1998.
- [9]Stehle G,Friedrich E A,Sinn H,etc.Hepatic uptake of a modified low molecular weight heparin in rats.[J].1992.
- [10]王金泉,刘志红.糖皮质激素免疫抑制作用机理的新认识[J].1997,(2).