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Study on the Mechanism of Brain Histochemistry Change and Neurons Apoptosis of Old Rats With Meynert Nucleus Damaged
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1008-1372.2002.11.007
- Key Word:
- Alzheimer病;中枢胆碱能系统;NMDA;神经元凋亡;鼠
Abstract： 目的用兴奋性神经毒素N-甲基-D-天冬氨酸(NMDA) 损伤大鼠脑胆碱能神经元, 研究NMDA损伤对认知能力和神经元凋亡的影响及NO在其中的作用.方法用 NMDA定位注射损伤老化大鼠脑右侧Meynert核.测定损伤动物学习能力改变(Y型迷宫法),检测脑胆碱酯酶活性(分光光度法),并用免疫组化染色法观察动物脑皮层及海马一氧化氮合酶(NOS)及凋亡相关蛋白Bax、Bcl-2表达阳性神经元的变化情况.结果与对照组比较, 经NMDA损伤后老化大鼠显示学习能力及脑胆碱酯酶活性显著降低.(P＜0.01)NOS和Bax在额叶皮层和海马的表达阳性神经元数量均显著升高,而Bcl-2阳性神经元数量则显著减少.(P＜ 0.01).结论兴奋性毒素NMDA定位损伤脑Meynert核后,老化大鼠显示胆碱能系统损伤,学习能力障碍.损伤脑NOS酶表达增加,提示有NO过量生成.Bax过表达而Bcl-2表达降低,显示脑中诱导发生神经元凋亡.这与AD病人某些临床和病理特征基本一致.
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