You Position: Home > Paper
A Comparative Study on Cartilage Induction by Bone Morphogenetic Protein Mixed With Different Carriers in Canine Tracheal Autografts
( views:124, downloads:0 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1008-1372.2002.11.006
- Key Word:
- 气管/移植;骨形态发生蛋白质;载体;狗
Abstract： 目的以三种可溶性物质作为重组人骨形态发生蛋白-2(rhBMP-2 )的载体,寻求适合作为rhBMP-2在犬的自体气管移植段内诱发新生软骨的缓释载体.方法 32只犬随机等分4组,颈部切取5环气管段作为移植段.等量的rhBMP -2分别与葡聚糖、聚乙烯吡咯烷酮(PVP)和胶原复合,另设无载体作为对照,均注射入各移植段内.然后将移植段埋入自体的腹腔大网膜中.于术后4周处死动物取材,行大体及组织学观察.结果移植段固有软骨环被部分地吸收.PVP和胶原组软骨环间rhB MP-2复合物植入区有大量新生软骨形成,且以胶原组更明显.结论 PVP和胶原均可协助rhBMP-2诱发气管移植段软骨再生,胶原更适合充当rhBMP-2的缓释载体.
- [1]李亚非.四种可溶性载体对骨形态发生蛋白在小鼠体内诱导成骨活性的影响[J].1997,(2).
- [2]李小飞.气管代用品及气管移植研究的进展[J].1996,(11).
- [3]Keshavjee S,Kesten S,Boehler A,etc.Lymphocytic airway infiltration as a precursor to fibrous obliteration in a rat model of bronchiolitis obliterans.[J].1997,64(2).
- [4]李亚非.应用胃蛋白酶消化的牛腱胶原作为骨形态发生蛋白的缓释载体（动物实验）[J].1996,(1).
- [5]Minamide A,Hashizume H,Kawakami M,etc.Experimental spinal fusion using sintered bovine bone coated with type I collagen and recombinant human bone morphogenetic protein-2.[J].1999,24(18).
- [6]G, Hotz,G, Herr.Bone substitute with osteoinductive biomaterials--current and future clinical applications.[J].1994,23.
- [7]Shimizu Y,Yamamoto Y,Liu Y,etc.Immunosuppressant-free allotransplantation of the trachea: the antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment.[J].2000,120(1).
- [8]Okubo Yasunori,Bessho Kazuhisa,Fujimura Kazuma,etc.Osteogenesis by Recombinant Human Bone Morphogenetic Protein-2 at Skeletal Sites[J].2000.
- [9]田卫东.煅石膏与羟基磷灰石复合人工骨的实验研究[J].1998,(3).