You Position: Home > Paper
The Expression of MMP-2 in EAM and the Effects of Methylprednisolone
( views:214, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1008-1372.2002.09.003
- Key Word:
- 实验性自身免疫性肌炎;MMP-2;RT-PCR;免疫组织化学;动物模型,鼠
Abstract： 目的探讨MMP-2在EAM发病中的作用,以及甲基强的松龙对MMP-2基因mRNA和蛋白表达的影响.方法应用RT-PCR及免疫组织化学技术,检测MMP-2在EAM组、甲基强的松龙治疗组(EAMM组)和对照组大鼠外周血、脾脏、肌肉组织中的表达水平.结果⑴EAMM组大鼠发病程度低于EAM组,炎症细胞浸润和肌纤维坏死程度减轻.⑵MMP-2在EAM大鼠外周血、脾脏淋巴细胞中的表达上调; MMP-2蛋白在EAM组大鼠肌肉组织表达增加,与对照组比较,差异有显著性.EAMM组MMP-2 mRNA的表达及蛋白的表达均受到抑制,与EAM组比较差异有显著性.结论 MMP-2表达增加可能与EAM发病有关;甲基强的松龙能减轻EAM发病程度,其作用机理可能与MMP-2的表达下调有关.
- [1]Dalakas MC,Choi YC.Expression of matrix metalloproteinases in the muscle of patients with inflammatory myopathies.[J].2000,54(1).
- [2]Banda MJ,Goetzl EJ,Leppert D.Matrix metalloproteinases in immunity.[J].1996,156(1).
- [3]Toyka KV,Matsumoto Y,Schneider C,etc.Experimental autoimmune myositis in the lewis rat: lack of spontaneous T-cell apoptosis and therapeutic response to glucocorticosteroid application.[J].2000,107(1).
- [4]R. L. Dawkins.Experimental myositis associated with hypersensitivity to muscle[J].1965,90(2).
- [5]J F, Woessner.The family of matrix metalloproteinases.[J].1994,732.
- [6]Hilliard B,Fujioka T,Bhopale MK,etc.Pathogenesis of neuroimmunologic diseases. Experimental models.[J].1998,17(1).
- [7]H, Birkedal-Hansen.Proteolytic remodeling of extracellular matrix.[J].1995,7(5).