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Change of Blood Parathyroid Hormone Level in Hypoparathyroidism Treated by pcDNA3.1*PTH
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1008-1372.2002.03.003
- Key Word:
- 甲状旁腺激素;基因治疗;甲状旁腺功能低下症
Abstract： 目的研究pcDNA3.1·PTH 治疗甲状旁腺功能低下症时PTH的变化及PTH与pcDNA3.1·PTH剂量的关系.方法手术去掉家兔甲状旁腺,建立甲状旁腺功能低下症模型.将已构建的人甲状旁腺激素基因pcDNA3.1·PTH 以100μg/ml、300μg/ml、500μg/ml剂量注射模型家兔的骨骼肌内,测定注射后不同时间的血液PTH.结果注射pcDNA3.1·PTH 后24h模型家兔的PTH升高,7d达高峰水平,并持续2个月,以300μg/ml和500μg/ml为佳.结论人甲状旁腺激素基因治疗甲状旁腺功能低下是有效的,且以300μg/ml和500μg/ml为最佳剂量.
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