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The Molecular Mechanism of Glucocorticoid- Induced Insulin Resistance
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- Author:
- No author available
- Journal Title:
- JOURNAL OF CHINESE PHYSICIAN
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1008-1372.2001.09.007
- Key Word:
- 地塞米松;受体,胰岛素;蛋白质免疫印迹法;胰岛素受体底物
Abstract： 目的探讨糖皮质激素对原代培养老鼠脂肪细胞的糖转运活动及胰岛素信号肽的影响.方法分离的老鼠脂肪细胞在5mmol浓度葡萄糖加地塞米松0.3μmo1分别孵育2h、8h、16h和24h,然后测定糖的转运活动;用Western blotting测定细胞内胰岛素受体底物(IRS)1/2、肌醇磷脂-3-激酶85亚单位(p85)和蛋白激酶B(PKB)的蛋白表达.结果地塞米松治疗抑制了基础的和胰岛素刺激的葡萄糖转运活动,产生最大抑制作用时间分别为2h和24h,对IRS1的蛋白表达的抑制作用与培养的时间长短成正比.同时,它也削弱了p85和PKB的蛋白表达;但对IRS2呈现上调作用.结论糖皮质激素可以抑制葡萄糖的转运活动,诱导胰岛素抵抗.其机理可能与改变胰岛素信号肽的表达等因素有关.
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